BBa_K101012

BBa_K101012 Version 1

Component

This part has been discontinued.

Please consider using BBa_K101007 as a replacement.

Source: http://parts.igem.org/Part:BBa_K101012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jeremiah Riesberg

Date created: 2008-08-03 11:00:00

Date modified: 2015-05-08 01:08:42

Construct with TetR/p22cII promoter, RBS, RFP, Terminator

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K101012_sequence (Version 1)

Description

The promoter contains binding sites for both TetR and p22mnt. RBS is the ribosomal binding site. RFP is the red fluorescent protein, which is a reporter gene. The terminator will terminate transcription.

Notes

Majority of design considerations were dealt with during the design of the dually repressed promoter (K101000). A medium strength RBS was used to increase the affinity of this part for ribosomal binding. Additionally, this decision was made to use only a single terminator.

Source

Construct is composed of: TetR promoter (R0040), p22mnt promoter (R0073), RBS (B0032), RFP (J06504), and Terminator (B1006).

Components

Access	Instance	Definition
public	BBa_K101002	BBa_K101002
public	BBa_B0032	BBa_B0032
public	BBa_J06504	mCherry
public	BBa_B1006	BBa_B1006

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K101002	1,66	BBa_K101002
BBa_B0032	75,87	BBa_B0032
BBa_J06504	94,807	mCherry
BBa_B1006	816,854	BBa_B1006

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• isReplacedBy: BBa_K101007/1
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K101012/1

Member of these Collections

• iGEM Parts Registry