BBa_K1030004

BBa_K1030004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1030004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wei Huang

Date created: 2013-09-15 11:00:00

Date modified: 2015-05-08 01:08:46

J23104->tetA-asv+sfGFP

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1030004_sequence (Version 1)

Description

We use a synthetic constitutive promoter J23104 to drive the expression of tetA, and sfGFP. tetA could transport extracellular Nickel ion into cell and nickel is toxic to E coli. An asv degradation tag is added to the c-terminal of tetA. sfGFP is used to monitor tetA expression in real time using fluorescent plate reader, flow cytometer and microscope.

Notes

We use a series of synthetic constitutive promoter to tune the expression of tetA, which in turn tune the extracellular Nickel tolerance level. sfGFP is used to monitor tetA expression.

Source

J23104 and sfGFP are from iGEM, tetA is from pBR322.

Components

Access	Instance	Definition
public	BBa_J23104	BBa_J23104
public	BBa_B0034	BBa_B0034
public	BBa_J31006	tetA(C)b
public	BBa_I746916	BBa_I746916

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23104	1,35	BBa_J23104
BBa_B0034	44,55	BBa_B0034
BBa_J31006	64,1254	tetA(C)b
BBa_I746916	1261,1980	BBa_I746916

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1030004/1

Member of these Collections

• iGEM Parts Registry