BBa_K105023

BBa_K105023 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K105023

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Katja Karstens

Date created: 2008-10-25 11:00:00

Date modified: 2015-05-08 01:08:52

lac I - operator

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K105023_sequence (Version 1)

Description

This BioBrick is the lac I recognition site. It can be used for the construction of promoters which should be regulated by activators or repressors containing a lac I DNA binding domain ([[Part:BBa_K105006| BBa_K105006]]).

Notes

This BioBrick contains the consensus sequence plus 5 spacer nucleotides on each side. The spacer is directly followed by the restriction site without a base in between. So when you construct multiple operator sites from this basic part you will have 16 bp between two consensus sequences.

Source

from oligos

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
consensus sequence	6,20	feature/conserved, conserved_region

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K105023/1

Member of these Collections

• iGEM Parts Registry