BBa_K1088011

BBa_K1088011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1088011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andreas Kj??r

Date created: 2013-08-27 11:00:00

Date modified: 2015-05-08 01:09:06

B. subtilis dxs (lac promoter without lac inhibitor)

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K1088011_sequence (Version 1)

Description

This part has the B. sub dxs (BBa_K1088000) controlled by the common Lac promoter. Notice that there is no LacI in this system, which is required for regulation of the Lac promoter.

Notes

We forgot to acknowledge the need for high expression of LacI to keep the regulation of the lac promoter vaild.
The constructs where assembled with USER cloning, and therefore there are no scarsites between the parts. There are however inserted scarsites between the RBS and the coding region aswell as between the lac promoter and the RBS to improve translational efficiency

Source

The Lac promoter derives from E. coli. The Dxs protein is from B. sub

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_K1088021	BBa_K1088021
public	BBa_J15001	BBa_J15001
public	BBa_K1088000	dxs (B. su

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_K1088021	201,206	BBa_K1088021
BBa_J15001	207,216	BBa_J15001
BBa_K1088000	217,2118	dxs (B. su

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1088011/1

Member of these Collections

• iGEM Parts Registry