Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Toulouse
Date created: 2013-09-19 11:00:00
Date modified: 2015-05-08 01:09:21
heme oxygenase (ho1), ferredoxin oxidoreductase (PcyA) and tetracycline repressor (TetR) under const
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1132013_sequence (Version 1) |
Description
Two genes required for phycocyanobilin (PCB) biosynthesis and the coding region for the TetR protein with Ribosome Binding Site (RBS) under a strong promoter. The Part:BBa_K1132012 was inserted behind BBa_K608003, a strong promoter with a medium RBS.ho1 produces biliverdin IXalpha, it represents the first of two steps in PCB biosynthesis. PcyA converts biliverdin IXalpha to PCB. PCB associates with EnvZ fusion protein, Cph8 (Part:BBa_I15010), to transduce a light signal into a genetic response.
TetR binds to the p(TetR) (Part:BBa_R0040) and inhibits its operation. It regulates the light sensor Cph8 (Part:BBa_K1132015). aTc (anhydrotetracycline) binds to TetR and allows Cph8 production. Both of these two parts construct the red light responsive system, under tetR regulation.
Notes
Two genes required for phycocyanobilin (PCB) biosynthesis and the coding region for the TetR protein with Ribosome Binding Site (RBS) under a strong promoter. The Part:BBa_K1132012 was inserted behind BBa_K608003, a strong promoter with a medium RBS.ho1 produces biliverdin IXalpha, it represents the first of two steps in PCB biosynthesis. PcyA converts biliverdin IXalpha to PCB. PCB associates with EnvZ fusion protein, Cph8 (Part:BBa_I15010), to transduce a light signal into a genetic response.
TetR binds to the p(TetR) (Part:BBa_R0040) and inhibits its operation. It regulates the light sensor Cph8 (Part:BBa_K1132015). aTc (anhydrotetracycline) binds to TetR and allows Cph8 production. Both of these two parts construct the red light responsive system, under tetR regulation.
Source
later| Access | Instance | Definition |
| public public | BBa_K608003 BBa_K1132012 | BBa_K608003 BBa_K1132012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K608003 BBa_K1132012 | 1,56 63,2465 | BBa_K608003 BBa_K1132012 |