Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Adrien Rapeaux
Date created: 2013-09-22 11:00:00
Date modified: 2015-05-08 01:09:23
Plac-RBS-KillerRed (IPTG-inducible, pQE30 plasmid format)
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1141001_sequence (Version 1) |
Description
This biobrick contains the PLac and RBS sequences from QIAGEN's pQE30 plasmid in front of the red fluorescent protein KillerRed. KillerRed has an additional function as a photosensitizer and the protein can be used to kill cells if illuminated by strong light while expressing KillerRed. KillerRed's presence in a culture can be seen (red colour similar to RFP), and the level of fluorescence corresponds to the amount of protein. Fluorescence peaks are 535 nm for absorption and 610 nm for emission. The protein dimerizes with itself and is not suitable for protein fusions.Notes
NoneSource
The source of this part is the pQE30 plasmid from QIAGEN (for PLac-RBS) and the KillerRed sequence from INVITROGEN. KillerRed was originally engineered from the anm2cp anthomedusa chromoprotein by Maria E. Bulina et al.| Sequence Annotation | Location | Component / Role(s) |
| PLac Eco RI RBS ATG Bam HI KillerRed 6x Histidine | 1,81 82,87 95,98 109,111 139,144 145,861 121,138 | promoter feature/promoter sequence_feature feature/dna ribosome_entry_site feature/rbs feature/start start_codon feature/dna sequence_feature CDS feature/protein tag feature/tag |