BBa_K1212014

BBa_K1212014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1212014
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Amy Soon
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:41

pBAD+Riboswitch1+TAL1



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1212014_sequence (Version 1)

Description

TAL repressor 1 expression under the control of a pBAD promoter and a theophylline riboswitch inducible by arabinose and theophylline.

Notes

There cannot be a scar between the end of the riboswitch and the start codon of the TAL repressor.

Source

The pBAD is a part from the registry. The riboswitch is from the Gallivan Lab's paper Synthetic riboswitches that induce gene expression in diverse bacterial species. by Topp et al. in Appl Environ Microbiol. The TAL repressor is from the Segal Lab at the UC Davis Genome Center. See their paper Quantitative Analysis of TALE-DNA Interactions Suggests Polarity Effects. by Meckler et al. submitted to Nucleic Acids Res.

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1212014/1