MarA

BBa_K1230000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1230000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andr??s Ferri??o Iriarte

Date created: 2013-09-07 11:00:00

Date modified: 2015-05-08 01:09:44

AcrAB-TolC multidrug efflux transport system activator from E. coli

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1230000_sequence (Version 1)

Description

The MarA protein acts as a transcriptional activator in E. coli, it allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to organic solvents, dyes, detergents, antibiotics such as chloramphenicol, tetracycline, novobiocin, erythromycin, fusidic acid and cloxacillin, as well as to cationic antimicrobial peptides, such as LL-37, HNP-2 and HBD-1.

Notes

Silent mutation (G->C) at base 132 to remove AarI and BspMI sites.

Source

Synthesis from genscript

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
MarA	1,393	feature/cds, CDS
stop	388,393	stop_codon, feature/stop
start	1,3	start_codon, feature/start
G to C mutation	132,132	point_mutation, feature/s_mutation

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1230000/1

Member of these Collections

• //regulation/positive
• iGEM Parts Registry