BBa_K1315008

BBa_K1315008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1315008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dimitrios Michailidis
Date created: 2014-09-25 11:00:00
Date modified: 2015-05-08 01:09:50

The cblD promoter from Burkholderia



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1315008_sequence (Version 1)

Description

This is a promoter that is activated by the OmpR-like two-component response regulator BCAM0228 (Bba_K1315007) in the BCAM0227-BCAM0228 sensor system. The Burkholderia diffusible signal (BDSF) is being picked up by the BCAM0227 sensor kinase that is hypothesised to eventually lead in involvement of BCAM0228. BCAM0228 is then activating cblD.

Notes

The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector

Source

The source of this part is Burkholderia cenocepacia J2315 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.

Sequence Annotation Location Component / Role(s)
cblD
1,250
promoter feature/promoter
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1315008/1