BBa_K136014

BBa_K136014 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K136014
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: alexandra bouaziz
Date created: 2008-10-24 11:00:00
Date modified: 2015-05-08 01:10:03

AracpBAD - gfp triparts



    • Details

    Types
    DnaRegion

    Roles
    Composite

    engineered_region

    Sequences BBa_K136014_sequence (Version 1)

    Description

    pBad is an E. coli promoter that is tightly controlled by:
    *inducer: L-arabinose.
    * repressor: AraC apparently acts as the repressor

    one study concluded that arabinose can change the conformation of araC and prevent it from successfully binding to and repressing pBad.
    pBad is followed by a GFP tripart in our construction .

    Notes

    AracpBAD was digested by SpeI -PstI (insert) and cloned into the psb2k3 that contain the gfp tripart .

    Source

    flgA promotor comes from E. coli K12 strain MG1655. gfp tripart comes from the registry.

    igem#experience
    None
     
    igem#sampleStatus
    Discontinued
    igem#status
    Deleted
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K136014/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
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