BBa_K136015

BBa_K136015 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K136015
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: alexandra bouaziz
Date created: 2008-10-24 11:00:00
Date modified: 2015-05-08 01:10:03

pBad/araC - GFP tripart



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K136015_sequence (Version 1)

Description

pBad is an E. coli promoter that is tightly controlled by:
*inducer: L-arabinose.
* repressor: AraC apparently acts as the repressor

one study concluded that arabinose can change the conformation of araC and prevent it from successfully binding to and repressing pBad.
pBad is followed by a GFP tripart in our construction .

Notes

AracpBAD was digested by SpeI -PstI (insert) and cloned into the psb2k3 that contain the gfp tripart .

Source

flgA promotor comes from E. coli K12 strain MG1655. gfp tripart comes from the registry.

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K136015/1