Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shoujie Sun
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:04
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1361004_sequence (Version 1) |
Description
This part contains the major subunit of curli fiber CsgA-his and its nucleator CsgB, in which CsgA-his is constitutive expression whereas CsgB is under the control of Pbad promotor. This part is designed to be used for immediate generate of large amount of curli fiber after adding of the inducer, and also can be used for purification of CsgA by Ni-NTA reign, or absorbing Au-NTA-Ni nano partical. This part cannot functioning alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.Notes
The modifying of CsgA was referring the supplementary of Lu, Michelle Y., et al. "Synthesis and patterning of tunable multiscale materials with engineered cells." (2014).Source
CsgB and CsgC were collected by PCR from genomic DNA of E coli. DH5alpha strain. CsgA-his was synthesized by GeneScrip.| Access | Instance | Definition |
| public public public public public public public | BBa_K206000 BBa_B0034 BBa_K1361997 BBa_B0015 BBa_K608003 BBa_K1361994 BBa_K1361993 | pBAD BBa_B0034 BBa_K1361997 BBa_B0015 BBa_K608003 BBa_K1361994 BBa_K1361993 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K206000 BBa_B0034 BBa_K1361997 BBa_B0015 BBa_K608003 BBa_K1361994 BBa_K1361993 | 1,130 139,150 157,612 621,749 758,813 820,1317 1324,1656 | pBAD BBa_B0034 BBa_K1361997 BBa_B0015 BBa_K608003 BBa_K1361994 BBa_K1361993 |