BBa_K1362999

BBa_K1362999 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1362999

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha

Date created: 2014-10-07 11:00:00

Date modified: 2015-05-08 01:10:06

iGEMHD tag

Details

• Types: DnaRegion
• Roles: tag; Tag
• Sequences: BBa_K1362999_sequence (Version 1)

Description

This Part adds ultimate coolness to you your part.
More seriously, it served us as a random sequence being a better overlap site for CPEC cloning than the RFC[10] suffix.

Notes

We aimed for a sequence without secondary structures, balanced GC content.

Source

Directly synthesized as an oligo as backtranslated with EMBOSS[1].

References

1. Rice, P., Longden, I. & Bleasby, A. EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet. 16, 276???7 (2000).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
stop	24,26	feature/stop, stop_codon
stop	21,23	feature/stop, stop_codon
iGEMHD	3,20	tag, feature/tag

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1362999/1

Member of these Collections

• iGEM Parts Registry