Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiajun Tan
Date created: 2014-10-01 11:00:00
Date modified: 2015-07-22 02:47:15
OprF(Val188)+GS linker+CBP
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1393000_sequence (Version 1) |
Description
The OprF is a major outer membrane protein of Pseudomonas aeruginosa. This protein functions as a nonspecific porin to allow the passage of small hydrophilic molecules, plays a structural role in maintaining cell shape and outer membrane integrity, and is required for growth under low osmolality.The structure of OprF has been proposed to consist of three domains, the N-terminal forming h-barrel structure, a loop or hinge region, and the C-terminal associated with peptidoglycan.Notes
Based on the predicted secondary structure and information found in the literature, we chose Val(188）as potential fusion sites for displaying CBP.CBP is the abbreviation of copper binding peptide made up of seven amino acid.In order to reduce the effect on the CBP activity，we added (G4S) linker between OprF and CBP .Source
The OprF gene is from Pseudomonas aeruginosa.| Sequence Annotation | Location | Component / Role(s) |
| OprF to Val188 CBP | 1,564 579,600 | feature/protein CDS non_covalent_binding_site feature/binding |