Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cristina Garcia-Timermans
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:17
Expression of agaA in E coli
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1403018_sequence (Version 1) |
Description
Name: BBa_K1403018Description: Biobrick submission of agaA expression construct
Resistances : Cm
Parts: Promoter - RBS - agaA - his tag
Design date: 11/June/2014
agaA is a gene present in Corynebacterium striatum and it is the main responsible for body odor according to the literature. It transforms 3-methyl-2-hexenoic acid into 3-hydroxy-3-methylhexanoic acid.
Detailed description of the agaA cosntruct:
- Promoter BBa_J23108 from the Anderson's promoter collection
- RBS from the RBS Calculator of Salis lab specific for E coli
- BioBrick Prefix + Scar from the iGEM Parts webpage
- agaA sequence codon optimized for E coli 12 (IDT online tool). We checked with Generious that there were no restriction sites for EcoRI, SpeI, XbaI and PstI.
- Histidine tag: 6 Histidines in C-terminal were added
-Stop codon
-BioBrick scar + BioBrick suffix from the iGEM Parts webpage
Notes
agaA sequence codon optimized for E coli 12 (IDT online tool). We checked with Generious that there were no restriction sites for EcoRI, SpeI, XbaI and PstI.Source
It is the agaA sequence codon optimized for E coli.| Access | Instance | Definition |
| public public public public | BBa_J23108 BBa_K1403010 BBa_K1403002 BBa_K128005 | BBa_J23108 BBa_K1403010 AgaA His |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23108 BBa_K1403010 BBa_K1403002 BBa_K128005 | 1,35 44,78 85,1282 1291,1308 | BBa_J23108 BBa_K1403010 AgaA His |