Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nandjafot MENDY
Date created: 2014-10-14 11:00:00
Date modified: 2015-05-08 01:10:18
P0 promoter-RBS SD001-sfGFP- Terminator B0015 - Pr promoter-DmpR
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_K1413002_sequence (Version 1) |
Description
This part consist on a sensor of phenolic compounds based on DmpR, a transcription factor of the Ntrc family. Found in Pseudomonas sp. strain CF 600, DmpR regulates expression from the Po promoter, which drives transcription from one single large operon for phenol degradation (dmpKLMNOPQBCDEFGHI). With GFP attached to P0 promoter, it is then possible to evaluate presence of phenol by fluorescence analysis, if DmpR is expressed. This part is basically composed of [P0 promoter + sfGFP + Pr promoter + DmpR] and allows sensing of phenolic compounds thanks to a constitutively expressed DmpR that will activate expression of GFP. We characterized this biosensor using phenol as effector.Notes
It has been obtained by performing a mutation of 3 nucleotideS within the sequence of RBS B0032 32.Source
This part derives from BBa_K1413001.| Sequence Annotation | Location | Component / Role(s) |
| P0 Promoter RBS JUNCTION RBS SD001 RBS JUNCTION sfGFP TERMINATOR SpeI/XbaI mixed site PR Promoter DmpR | 1,171 172,179 180,192 193,198 199,929 930,1058 1059,1066 1082,1155 1156,2847 | promoter feature/promoter feature/misc sequence_feature ribosome_entry_site feature/rbs sequence_feature feature/misc feature/cds CDS sequence_feature feature/misc feature/misc sequence_feature feature/promoter promoter sequence_feature feature/misc |