P43-spoVG

BBa_K143051 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K143051

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chris Hirst

Date created: 2008-09-29 11:00:00

Date modified: 2015-05-08 01:10:24

Promoter 43 and RBS spoVG for B. subtilis

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K143051_sequence (Version 1)

Description

Constitutive promoter 43(BBa_K143013) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis.

P43-spoVG can be used in the context of a Polymerase per second (PoPS) output generator.

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Notes

The sequence of P43 and RBS-spoVG were obtained from papers1 2 and the sequence synthesised by GeneArt.

Source

P43-spoVG was synthesised by GeneArt.

Components

Access	Instance	Definition
public	BBa_K143013	P43
public	BBa_K143021	RBS-spoVG

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K143013	1,56	P43
BBa_K143021	65,76	RBS-spoVG

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K143051/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• //chassis/prokaryote/mflorum
• iGEM Parts Registry