BBa_K1444009

BBa_K1444009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1444009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dan Ziemianowicz
Date created: 2014-10-07 11:00:00
Date modified: 2015-05-08 01:10:27

Composite promoter and consensus B.subtilis RBS - C1-434 x3



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1444009_sequence (Version 1)

Description

A composite part consisting of the constitutive promoter Pveg (BBa_K823003), followed by the copies of the repressible promoter C1-434 (BBa_R0052), and ending with the consensus RBS from B. subtilis (BBa_K090505).

This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pLacI repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed. The triplicate repressible promoter allows for stronger repression.

Notes

No interference with endogenous cell signalling.

Source

B. subtilis

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1444009/1