Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grzegorz Ścibisz
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:32
PmrB(WT) from Salmonella enterica
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1459016_sequence (Version 1) |
Description
Protein name:PmrBOther names:basS, parB
Gene name:basS
Source organism for the data:Salmonella enterica subsp. enterica serovar Typhimurium str. strain LT2 / SGSC1412 / ATCC 700720
UniProtKB signature:P36557/ Gene sequence RefSeq accession number:NC_003197.1
Protein sequence RefSeq accession number:NP_463157.1
Length:356 aa
Molecular mass:40,262 Da
Cellular localization:inner plasma membrane
Biological function:Signal transduction via kinase acivities
PmrB(LBT) is a engineered PmrB gene, where PmrB is a sensor histidine kinase present in the inner cell membrane of many species of bacteria, including E. coli and S. enterica. With a 30 amino acid periplasmic loop, it is capa ble of binding iron (III) and aluminium ions. The binding event induces a conformational change of the protein, which leads to ATP phosphate-derived autophosphorylation of the C-terminal cytoplasmic domain, followed by transfer of the phosphate group onto th e transcriptional regulator PmrA. As part of our project, the periplasmic iron/alumin ium-binding loop of the PmrB was substituted with a synthetic sequence - a lanthanide-binding ta g, intended to bind lanthanide ions, with terbium in particular. Such a binding event would then indu ce the aforementioned conformation change and phosphorylation of the PmrA, leading it to bind to the PmrC promoter, to allow for expression of the Green Fluorescent Protein - our reporter gene.
If you wish to study PmrA-PmrB system more closely, we suggest familiarising yourself with following papers:
[1] H. Liang, X. Deng, M. Bosscher, Q. Ji, M. P. Jensen, C. He, Engineering Bacterial Two-Component System PmrA/PmrB to Sense Lanthanide Ions, J.Am.Chem.Soc. 2013, 135, 2037−2039
[2] M. Wonsten, L. Kox, S. Chamnogpol, F. Soncini, E. Groisman, A Signal Transduction System that Responds to Extracellular Iron,Cell, Vol. 103, 113???125, September 29, 2000