Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pascal Sartor
Date created: 2014-10-01 11:00:00
Date modified: 2015-05-08 01:10:39
Galactose-induced gene 4 DNA binding domain (Gal4DBD)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1470002_sequence (Version 1) |
Description
This yeast protein is a positive transcriptional regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which are important for galactose import and conversion to glucose []. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.GAL4 is used to investigate gene expressions in several organisms (bacteria, plants, fruit flyes) [].
The protein is controlled by a strong promotor. Binding to UAS-G leads to the expression of the corresponding gene.
References
BRAND, AH., PERRIMON, N.: Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development. 1993 Jun;118(2):401-15.
TRAVEN, A., JELICIC, B., SOPTA, M.: Yeast Gal4: a transcriptional paradigm revisited. EMBO Rep. May 2006; 7(5): 496???499.