BBa_K1472613

BBa_K1472613 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1472613
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lau Tsz Kit
Date created: 2014-11-01 12:00:00
Date modified: 2015-05-08 01:10:39


9, ∆
12, ∆
15 desaturases with LacI promoter



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K1472613_sequence (Version 1)

Description

This protein generator is composed of the delta 9, delta 12 and delta 15 desaturase genes with an RBS upstream of each coding sequence and LacI regulated promoter. Desaturases introduce cis-carbon double bonds to the carbon chain of fatty acids. ∆9 desaturase introduces a double bond to stearic acid between carbon 9 and carbon 10 to produce oleic acid. ∆12 desaturase introduces a double bond between carbon 12 and carbon 13 of oleic acid so to produce linoleic acid. In the end, ∆15 desaturase converts linoleic acid into alpha-linolenic acid by adding a double bond between carbon 15 and carbon 16 (Figure 1).

Notes

The desaturase genes in plasmids were introduced into E. coli for amplification. The final construct includes iGEM provided RBS (BBa_B0034).

Source

All the desaturase sequences are derived from the Synechocystis sp. but optimised for the expression in E. coli.

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1472613/1