BBa_K1519123

BBa_K1519123 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1519123
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Raoul Martin, Drew Dunham
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:10:48

OsmY based secretion for purification



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_K1519123_sequence (Version 1)

Description

By inserting the DNA sequence of a desired protein in our part, you will be able to secret your protein, bind it to a Nickel column, and cleave off the desired protein from the tag in very few steps. The OsmY-tag will allow you to have your protein fold in the periplam and form disulfide bonds. Once folded OsmY also allows the portein to be secreted into the media, which contains significantly less portion of endesired protein. The media containing the construct can there be run through a nickel column for further purification. Once elluted from the column, one can add TEV-His protease to cleave the tag off the desired protein. By re-running the protein in a nickel column, the desired portein will elute directly, while the tag and the TEV-His will stay bound to the column.

Notes

None.

Source

OsmY comes from the MG1655 E.coli strain.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1519123/1