Types | DnaRegion
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Roles | polypeptide_domain
Protein_Domain
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Sequences | BBa_K157008_sequence (Version 1)
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Description
N-terminal fragment (Amino acids1-156) of the monomeric[5] yellow fluorescent protein variant ???Venus??? [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157007 (Split-Venus-cYFP) generates the complete, working YFP ???Venus???, whereas combination with the C-terminal fragment (Part Bba_K157005) of the monomeric cyan fluorescent Protein ???CeruleanR206K??? (Part Bba_I757009) results in green fluorescence[2].See "Part Design" for references.
Notes
Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
Source
Gene synthesis by GeneArt, optimized for expression in homo sapiens.