Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Robert Marsden
Date created: 2015-09-07 11:00:00
Date modified: 2016-01-25 11:30:54
pRha + CsgA with His-tag & pTet + RFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1583107_sequence (Version 1) |
Description
Upon induction with rhamnose, CsgA with a N-terminal His tag is expressed. These protein monomers can aggregate to form amyloid nanofibres.In order to better visualize the individual cells in the biofilm, we added the constitutive expression of RFP to our plasmid in a different operon. For our design, we took advantage of the double terminator present in the biobrick I13521 by placing it in front of our genes.
Notes
For our design, we took advantage of the double terminator present in the biobrick I13521 by placing it in front of our genes.Source
The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).| Access | Instance | Definition |
| public public | BBa_I13521 BBa_K1583101 | BBa_I13521 BBa_K1583101 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13521 BBa_K1583101 | 1,923 932,1591 | BBa_I13521 BBa_K1583101 |