BBa_K1598003

BBa_K1598003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1598003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Napiorkowska
Date created: 2015-08-26 11:00:00
Date modified: 2015-08-28 10:29:27

RBS-ChAT-6xHis-Terminator



    • Details

    Types
    DnaRegion

    Roles
    Generator

    engineered_region

    Sequences BBa_K1598003_sequence (Version 1)

    Description

    This part consists of B0034 RBS, human choline acetyltransferase (CHAT) gene with C-terminal His tag, and B0015 terminator.

    Notes

    - human CHAT gene (UniProt accession no: P28329) was codon-optimized for E.coli and provided with C-terminal 6xHis tag
    - due to its length, the sequence was synthesized as two gBlock fragments with 30 bp overlap between each other and with 20 bp overlaps with respective PSB1C3 ends
    - the construct was cloned into PSB1C3 using Gibson Assembly

    Source

    DNA synthesis

    Sequence Annotation Location Component / Role(s)
    RBS B0034
    CHAT
    Terminator B0015
    		1,12
    19,1932
    1941,2069
    		ribosome_entry_site feature/rbs
    CDS feature/cds
    feature/stop stop_codon

    igem#experience
    None
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1598003/1
     

    Attachments

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