CYP11A1

BBa_K1598008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1598008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yash Mishra
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-21 03:58:55

J23101 promoter + RBS + Human FDXR + RBS + Human FDX1 + RBS + Human CYP11A1 + rrnb double terminator



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1598008_sequence (Version 1)

Description

Also called Cytochrome P450scc, Cholesterol Side Chain Cleavage Enzyme (P450scc) is a mitochondrial enzyme that converts cholesterol into pregnenolone, which is a neurosteroid as well as the precursor to a variety of steroid hormones and neurotransmitters. The gene that encodes it is CYP11A1. Furthermore, the catalysis involves 3 monooxygenase reactions, which requires two electron transport proteins: ferrodoxin (FDX1) and ferredoxin reductase (FDXR). Our biobrick consists of the iGEM prefix followed by the J23101 constitutive promoter, RBS, FDXR gene, RBS, FDX1 GENE, RBS, CYP11A1 gene, rrnb double terminator, and the iGEM suffix. This will be ligated onto a PSB1C3 backbone.

Emerging preclinical and clinical evidence suggests that pregnenolone may be a promising novel therapeutic candidate in schizophrenia. It has also been shown to reduce the side effects of benzodiazepines. Furthermore, cholesterol side chain cleavage is the 1st step in the biosynthesis of steroids such as testosterone, progesterone, cortisol, estrogen, etc. Therefore, this biobrick has a plethora of applications, and is in a way several biobricks in 1.

Notes

We had to make sure that that the genes for Cytochrome P450scc and all it's electron transport proteins were included in the biobrick and came after ribosome binding sites. The biobrick was broken into 5 parts for quick synthesize and assembled using Golden Gate assembly. Hence, each part was made compatible for golden gate assembly, and had overhangs for SapI.

Source

http://www.ncbi.nlm.nih.gov/nuccore/153218645?report=fasta
http://www.ncbi.nlm.nih.gov/nuccore/16877631?report=fasta
http://www.ncbi.nlm.nih.gov/nuccore/39645178?report=fasta
http://parts.igem.org/cgi/partsdb/puttext.cgi
http://parts.igem.org/cgi/partsdb/puttext.cgi

Sequence Annotation Location Component / Role(s)
Human FDX1 Gene
BBa_J23101
Human FDXR Gene
BBa_J34801
BBa_J34801
BBa_J34801
Human CYP11A1 Gene
rrnb Double Terminator preceded by some random bases
1562,2117
1,33
58,1534
39,51
1543,1555
2126,2138
2151,3716
3717,3910
CDS feature/cds
promoter feature/promoter
feature/cds CDS
ribosome_entry_site feature/rbs
feature/rbs ribosome_entry_site
feature/rbs ribosome_entry_site
feature/cds CDS
feature/stop stop_codon
igem#experience
Works
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1598008/1