Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Johanna Chesnel
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-22 07:13:37
T7 promoter - HisTag- NanoLuc - T7 terminator
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1616023_sequence (Version 1) |
Description
NanoLuc (Nluc) is a novel engineered luciferase coupled with a novel coelenterazine analogue: Furimazine. When both are coupled, we obtain a much brighter luminescence than Fluc or Rluc and it is known to work exceptionally well as a reporter.NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence.
Thermal stable enzyme
NanoLuc retains activity following 30 min incubation at 55 ??C.
Active over broad pH range
NanoLuc is fully active between pH 7-9 and retains significant activity at pH 5-7.
Monomeric enzyme
NanoLuc facilitates use as transcriptional reporter or fusion partner.
Notes
Presence of XbaI site.Source
NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence.| Access | Instance | Definition |
| public public public | BBa_K1616028 BBa_K1616029 BBa_K1616027 | BBa_K1616028 BBa_K1616029 BBa_K1616027 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1616028 BBa_K1616029 BBa_K1616027 | 1,19 20,633 634,762 | BBa_K1616028 BBa_K1616029 BBa_K1616027 |