Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mustafa Yılmaz
Date created: 2015-09-12 11:00:00
Date modified: 2015-09-18 07:12:27
Tev Protease, single S219V mutation in the internal cleavage site
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1639008_sequence (Version 1) |
Description
TEV protease (also called Tobacco Etch Virus nuclear inclusion a endopeptidase) is a highly sequence-specific cysteine protease from Tobacco Etch Virus (TEV).The consensus for these native cut sites is ENLYFQ\S where ???\??? denotes the cleaved peptide bond. One of the main uses of this protein is for removing affinity tags from purified proteins. The reason for the use of TEV protease as a biochemical tool is its high sequence specificity. This specificity allows for the controlled cleavage of proteins when the preference sequence is inserted into flexible loops. It also makes it relatively non-toxic in vivo as the recognized sequence scarcely occurs in proteins.[1]Notes
This part contains an additional BamHI restriction site at beginning of Tev Protease sequence and a XhoI restriction site at the end.Source
Tobacco etch virus (TEV).| Sequence Annotation | Location | Component / Role(s) |
| ATG Start Codon Tev Protease Stop Codon S219V | 9,11 12,737 738,740 666,668 | feature/start start_codon CDS feature/cds stop_codon feature/stop sequence_alteration feature/mutation |