BBa_K1641013

BBa_K1641013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1641013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Pai Li

Date created: 2015-09-09 11:00:00

Date modified: 2015-09-10 02:50:51

SloxM1, modified recognition site of recombinase Scre

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_K1641013_sequence (Version 1)

Description

This is a recognition site of Cre-like recombinase Scre, that is "CTCGTGTCCGATA ACTGTAAT TATCGGACACGAG". The original recognition site of Scre is SloxP, "CTCGTGTCCGATA ACTGTAAT TATCGGACATGAT", contains ATG that might be used as Met to start translation. So two mutation is conducted to avoid ATG and although this generates slight mismatch, this will not significantly reduce the activity of Scre on this site.

This recognition site is not homologous with LoxP and hence will not cross-react with Cre or other common recombinases.

Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. The scar will be "ACTAGA" safely without cutting activity and start code. with This deletion do not interfere the usage of the brick.

Notes

SloxM1, a mutation of SloxP, eliminates ATG inside the sequence and avoids unexpected translation.

Source

De novo synthesis.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1641013/1

Member of these Collections

• iGEM Parts Registry