Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jianheng Liu
Date created: 2015-09-12 11:00:00
Date modified: 2015-09-13 06:27:34
pBAD(forward)-FRT(forward)-LoxP(reverse)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1641200_sequence (Version 1) |
Description
pBAD forward promoter+recombinase Flpe recognition site (forward)+recombinase Cre recognition site (reverse).Used in iGEM15_SYSU_China project.
Notes
This sequence has two primer sites and a restriction enzyme site between FRT and loxP for further testing in our experiment.Two primers are:
GCACCTTGACTCTGACAATCCT (forward)
GCCTGTGCTAATGGTGATGACT (reverse)
they both have a high annealing temperature (about 58C). They can be used as qPCR primers.
The restriction enzyme sites are HindIII and BanI
Source
Sequence was synthetized by IDT.| Access | Instance | Definition |
| public public public | BBa_I13453 BBa_J61020 BBa_K1641219 | BBa_I13453 FRT BBa_K1641219 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13453 BBa_J61020 BBa_K1641219 | 1,130 139,172 181,214 | BBa_I13453 FRT BBa_K1641219 |