Types | DnaRegion
|
Roles | CDS
Coding
|
Sequences | BBa_K1669004_sequence (Version 1)
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Description
This part includes the BdhB gene from Clostridium acetobutylicum (NCBI: CAP0163) with a C-terminal His-tag, which has been codon optimized for expression in E. coli.
This is the gene coding the butanol dehydrogenase enzyme, that is in Clostridium acetobutylicum responsible for conversion of butyryl-CoA to butanol.
The sequence contains a C-terminal His-tag for purification of the protein using the immobilized-metal affinity chromatography and detected with the anti-His antibodyes.
Notes
C-terminal His-tag.
Source
Sequence retrieved from GenBank and codon optimized for expression in E. coli