BBa_K1717000

BBa_K1717000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1717000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Our Lady of the Snows Catholic Academy 2015
Date created: 2015-09-12 11:00:00
Date modified: 2015-09-18 11:16:51

Keratinase A under control of LacI promoter.



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1717000_sequence (Version 1)

Description

This part is a composite of an IPTG-inducible promoter with RBS (BBa_J04500) and a custom-synthesized KERATINASE A expressing plasmid. The coding sequence for Keratinase A was originally isolated in Bacillus, we have now optimized for expression in E.coli. To do this, the signal peptide has been changed.

Notes

The bacteria that we are using is a strand of e-coli called K- 12. This strain has a negative granam making it opposite to the gram-positive bacter (Bacillus Licheniformis) the Keratinase that we chose to utilize for our project was found in. After extensive research and looking at the projects of chicago, sheffield and Taiga we learnt that the different ganaam is what causes the issues with the secretion of the keratinase as it gets stuck in the periplasm due to the fact that the signal peptides of the keratin can???t be read by the E. coli. After considering all of this we learned that we must completely remove the gram positive signal sequence, in order to fix this problem. The gram positive signal sequence was completely removed and in its place is PelB (BBa_J32015).

Source

Found on the following website: http://www.uniprot.org/uniprot/Q53521.

Access Instance Definition
public
public
BBa_J04500
BBa_K1717171
BBa_J04500
KERA
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1717000/1