BBa_K1725431

BBa_K1725431 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1725431
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrey Filipov
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-08 01:22:59

J23110 + RBS + UirR + B0015 + PlsiR + GFP



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1725431_sequence (Version 1)

Description

The UV intensity response regulator coding sequence was positioned downstream of J23110, a constitutive promoter family member, and upstream of the double terminator B0015. In this part the promoter associated with UirR was also added, alongside with GFP, so that activity may be reported.

Notes

J23110, UirR, and B0015 were ligated to Plsir and GFP after restriction that was followed by an Azure-A-based gel extraction.

Source

UirR and Plsir come from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1725431/1