Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Żardecka
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 07:29:42
sfGFP under melibiose promoter (improved 5'UTR)
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1741004_sequence (Version 1) |
Description
Twice higher expression has been obtained by 5???UTR editing ??? removal of potential secondary structure which involved RBS and a better positioning of RBS ??? 7 nt upstream AUG start codon. The modified 5???UTR results in about twice higher expression upon melibiose addition to the M9 or 2xLB medium. The promer is still weaker than araBAD or rhaBAD, so can be a promoter of choice if lower expression level is necessary for efficient folding or secretion of a recombinant proteinNotes
5'UTR editing.Source
All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.| Sequence Annotation | Location | Component / Role(s) |
| MelR binding site 1 CRP binding site MelR binding site 1 -35 signal -10 signal improved 5'UTR RBS His-tag sfGFP two consecutive STOP codons | 28,45 48,63 66,84 102,107 125,129 138,164 151,156 165,194 195,908 909,914 | feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/rbs ribosome_entry_site tag feature/tag CDS feature/protein stop_codon feature/stop |