BBa_K175043

BBa_K175043 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K175043
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tim_Weenink
Date created: 2009-10-10 11:00:00
Date modified: 2015-05-08 01:10:59

Inducible Self Destructive Plasmid + inducible GFP-LVA reporter



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_K175043_sequence (Version 1)

Description

Inducible Self Destructive Plasmid, consisting of IPTG inducible I-SceI homing endonuclease + inducible GFP-LVA reporter gene with an I-SceI restiction site integrated into the sequence between the promoter and the RBS. And an additional one between the GFP coding sequence and the TetR generator sequence.

Notes

Initial design of the Self Destructive Plasmid. However, expression of the reporter gene did not seem to work after induction with anhydrotetracyclin.

Source

See basic parts

igem#experience
Fails
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K175043/1