BBa_K176219

BBa_K176219 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K176219
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danqian Liu, Zongxiao He, Hao Jiang
Date created: 2009-10-07 11:00:00
Date modified: 2015-05-08 01:11:04

cell density->GFP: pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GFP



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_K176219_sequence (Version 1)

Description

GFP = (Cell Density * pCon 0.15) + Input AHL

High level GFP inhibits cell growth.

This part is both a cell density reporter and a cell density controller.

Notes

Input: AHL

Output1: AHL

Output2: GFP

Output3: Cell Density

Design:

pCon 0.15(K176008) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.

pCon 0.36(K176009) generates LuxR.

pLux/Tet(K176000) generates Output2 GFP, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc. High level GFP inhibits cell growth to control Output3 Cell Density.

Source

K176022 K176126

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BBa_K176219/1