BBa_K1835000

BBa_K1835000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1835000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anthony Ciesla

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-17 03:04:36

Empty gRNA Scaffold

Details

• Types: DnaRegion
• Roles: Other; sequence_feature
• Sequences: BBa_K1835000_sequence (Version 1)

Description

Using the restriction enzyme BsaI, an opening will be made to insert annealed oligonucleotides that can code your gRNA.

Notes

The BsaI sites were devised with enough space to avoid interference and designed to cut themselves out from the produced backbone.

Source

The part has been in use in our lab originally from the Luciano Marrafini Lab originating from S. Peumoniae.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Repeat	20,55	feature/stem_loop, stem_loop
Repeat Loop	86,122	stem_loop, feature/stem_loop
gRNA Oligos Binding Site	53,56	start_codon, feature/start
gRNA Oligos Binding Site	82,85	feature/stop, stop_codon

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1835000/1

Member of these Collections

• iGEM Parts Registry