Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhao Jingyu
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 01:25:38
Luciferase
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1891002_sequence (Version 1) |
Description
It encodes whole luciferase under the control of pBAD promoter. And we add arabinose until its final concentration is 1mmol/L, incubate for 6h(37??C, 250rpm). We can detect its activity by add its substrate fluorescein.Notes
The DNA sequence of luciferase gene is from NCBI (Genebank:M65067.1). A pBAD promoter(BBa_K206000) and a RBS(BBa_B0034) are added upstream the functional gene to produce this enzyme. Lociferase-pSB1C3 plasmid is transformed into E. coli strain transB (DE3) to express the protein. It can show fluorescence when we add its substrate fluorescein.Source
We get this gene sequence from NCBI (Genebank:M65067.1), and then we get the DNA fragment from a synthesis company.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K206000 BBa_B0034 luciferase BBa_B0015 | 1,130 131,142 143,2282 2283,2411 | feature/promoter promoter feature/rbs ribosome_entry_site feature/cds CDS stop_codon feature/stop |