Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhao Jingyu
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 08:58:12
alpha-tubulin+N-Luciferase
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1891005_sequence (Version 1) |
Description
This part expresses alpha-tubulin- N-Luciferase fusion protein . The C-terminal of alpha-tubulin links to the N-terminal of N-Luciferase. We connected this two gene fragment using fusion PCR. And then we cloned it into pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of this fusion protein.Notes
We connected this two gene fragments using fusion PCR. First we acquired alpha-tubulin gene fragment and N-Luciferase gene fragment. And then we redesigned a antisense primer of alpha-tubulin and a sense primer of N-Luciferase with a linker about 42bp. And then doing fusion PCR.Source
Alpha-tubulin comes from human breast cancer cell line Mcf-7 cDNA using PCR. N-Luciferase comes from pCambia1300-N-Luciferase plasmid.| Sequence Annotation | Location | Component / Role(s) |
| alpha-tubulin N-luciferase linker | 1,1356 1399,2646 1357,1398 | sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature |