Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xinyi Liu
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-11 06:31:46
The SNAP-mCHERRY UNIT
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1926022_sequence (Version 1) |
Description
This part is the SNAP-mCHERRY UNIT of cyclebow including a SNAP UNIT and a mCHERRY UNIT. The SNAP UNIT, which can be cut off by CRE, includes an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The mCHERRY UNIT, which can be cut off by VIKA, includes a degradation tag named DBOX, a fluorescent gene mCherry and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox.You may use this part to:
1) Test the cut-off efficiency of recombinase CRE and VIKA;
2) Estimate the time needed for genome repair because mCherry can only be expressed after the cut off of SNAP UNIT and genome repair;
3) Fast cut off this part by transient transfecting recombinase gene into nucleus;
4) When there is no CRE, you can use this part as the SNAP UNIT, which can help localize proteins in vivo with BLOCK and dye from NEB company.
Notes
NoneSource
The SNAP-tag?? was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1. The sequence of Vox and DBOX was retrieved from Addgene and got through denovo synthesis. mCHERRY was got from commercial plasmid: pentry dcas9 mcherry bira.| Access | Instance | Definition |
| public public | BBa_K1926011 BBa_K1926013 | BBa_K1926011 BBa_K1926013 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1926011 BBa_K1926013 | 1,788 789,1704 | BBa_K1926011 BBa_K1926013 |