Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oumnia KARIM
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-17 12:38:48
Epsilon/MazF toxin and antitoxin in pSBBS0K-mini
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1937010_sequence (Version 1) |
Description
(Chassis B.subtilis, carrier plasmid pSBBS0K)Length: 6855 bp
Background:
This Epsilon/MazF part is one of the two components of a double toxin-antitoxin system. The part was designed to be carried on a plasmid while its counterpart MazE/Zeta was carried on another. Unfortunately, only Epsilon/MazF part was successfully cloned in E. coli.
This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)
Notes
Epsilon and MazF are under the control of the promoter Pveg (constitutive promotor for B. subtilis) followed by a strong RBS. A theophylline-dependent riboswitch was used to prevent the MazE toxin expression during the cloning steps in presence of theophylline (Topp and Gallivan, 2008). Indeed, with an expressed toxin, it would be impossible to clone the Biobrick in E. coli.SacII and KpnI are the restriction sites placed respectively before and after the riboswitch so that this fragment can easily be replaced depending on the control we want to apply to the bacteria. The construction was created by integrating the EcorI-PstI fragment from BBa_K1937009 in the pSBBS0K-miniplasmid (BBa_K1937001).Source
The gene Epsilon codes for a toxin countered by the anti-toxin Zeta (Zielenkiewicz and Cegłowski, 2005 ; Mutschler et al., 2011). The gene MazF encodes the anti-toxin for the MazE toxin (Bravo et al., 1987, Zhang et al., 2005, Wang et al., 2013).| Access | Instance | Definition |
| public public | BBa_K1937001 BBa_K1937009 | BBa_K1937001 BBa_K1937009 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1937001 BBa_K1937009 | 1,5857 5866,6869 | BBa_K1937001 BBa_K1937009 |