Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ibrahim Al-Masoud
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-19 03:02:30
P-45 Promoter Phytobrick
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1968007_sequence (Version 1) |
Description
A widely used strong constitutive promoter from Corynebacterium (Patek et al., 1996) that works with related gram positive actinobacteria, such as Rhodococcus. We have this part in pUPD2, a MoClo-compatible level 0 vector with no illegal sites for MoClo assembly standard, GoldenBraid assembly standard and iGEM???s pairwise assembly standard.Notes
The part was integrated in reverse and we advise the user to use the reverse complement sequence for downstream applications.Source
This part was extracted through PCR from pSRKBB-empty vector (Addgene #59449) and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500 or pUPD2). The sequence of this part was confirmed by Sanger Sequencing.| Sequence Annotation | Location | Component / Role(s) |
| Phytobrick Adapter Site A Phytobrick Adapter SiteB P-45 Promoter | 1,4 151,154 5,150 | feature/misc sequence_feature sequence_feature feature/misc promoter feature/promoter |