Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuli Liang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-19 07:38:06
dszBBAC with tac promoter and dszD with lac promotor induced by IPTG
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1972014_sequence (Version 1) |
Description
We constructed dszBCAD genes with T7 promoter and IPTG controled system and choose E.coli as our host cell.T7 RNA polymerase will not be expressed without IPTG. When IPTG is added to 1 mM, T7 RNA polymerase will be expressed and dszBCAD genes with T7 promoter will be greatly expressed by the host cell. The four enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway.Each single biodesulfurization enzyme gene was synthesized by Generay and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)
Notes
Not yet.Source
Each single biodesulfurization enzyme gene was synthesized by Generay and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)The T7 RNA polymerase controled system is PCRed from E.coli Bl21.
| Access | Instance | Definition |
| public public public | BBa_K1972008 BBa_K1972005 BBa_K1972013 | BBa_K1972008 BBa_K1972005 BBa_K1972013 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1972008 BBa_K1972005 BBa_K1972013 | 1,1307 1316,3085 1316,7367 | BBa_K1972008 BBa_K1972005 BBa_K1972013 |