BBa_K199194

BBa_K199194 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K199194

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Todd Eckdahl

Date created: 2010-09-05 11:00:00

Date modified: 2015-05-08 01:11:21

TT-pLux-RBS-LuxI 2 sites-RBS-GFP-RBS-RFP-pLacI-RBS-LuxR

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K199194_sequence (Version 1)

Description

pLacI drives LuxR gene, producing LuxR protein for activation of pLux promoter. pLux drives LuxI gene, producing enzyme to make 3OC6, required for LuxR activation. The circuit has amplification by the LuxI enzyme and positive feedback. pLux also drives GFP gene, followed by RFP gene. The LuxI gene is modified with restriction sites for AscI and AsiSI, for introduction of frameshift mutations.

Notes

Introduction of restriction sites for AscI and AsiSI required changing several amino acids and retaining the reading frame.

Source

Registry intermediates

Components

Access	Instance	Definition
public	BBa_S04424	BBa_S04424
public	BBa_S04473	BBa_S04473

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_S04424	1,845	BBa_S04424
BBa_S04473	854,3478	BBa_S04473

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K199194/1

Member of these Collections

• iGEM Parts Registry