BBa_K1994016

BBa_K1994016 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1994016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Liam Carroll

Date created: 2016-09-25 11:00:00

Date modified: 2016-10-19 01:26:31

sgRNA with 5' golden gate adapter and MS2 coat protein binding site

Details

• Types: DnaRegion
• Roles: RNA; mature_transcript_region
• Sequences: BBa_K1994016_sequence (Version 1)

Description

For use in CRISPR/Cas9 systems. Contains golden gate adapter at 5' end for inserting target sequence. Contains a MS2 coat protein binding site in the middle.

Notes

No special considerations

Source

Synthetic

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
20nt golden gate adapter	1,20	sequence_feature, feature/misc
dCas9 handle	21,62	feature/stem_loop, stem_loop
truncated S. pyrogenes terminator	63,96	feature/stem_loop, stem_loop
5bp linker	97,101	feature/misc, sequence_feature
MS2 handle	102,126	feature/stem_loop, stem_loop
5bp linker	127,131	feature/misc, sequence_feature
aspA terminator	132,172	feature/misc, sequence_feature

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1994016/1

Member of these Collections

• //function/crispr/cas9
• iGEM Parts Registry