BBa_K1994017

BBa_K1994017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1994017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Liam Carroll

Date created: 2016-09-25 11:00:00

Date modified: 2016-10-19 01:34:26

sgRNA with 5' golden gate adapter and PP7 protein binding site

Details

• Types: DnaRegion
• Roles: RNA; mature_transcript_region
• Sequences: BBa_K1994017_sequence (Version 1)

Description

For use in CRISPR/Cas9 systems. Contains golden gate adapter at 5' end for inserting target sequence. Contains a PP7 coat protein binding site in the middle.

Notes

No special considerations

Source

Synthetic

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
20nt golden gate adapter	1,20	sequence_feature, feature/misc
dCas9 handle	21,62	feature/stem_loop, stem_loop
truncated S. pyrogenes terminator	63,96	feature/stem_loop, stem_loop
10bp linker	97,107	feature/misc, sequence_feature
PP7 handle	108,139	feature/stem_loop, stem_loop
10bp linker	140,150	sequence_feature, feature/misc
aspA terminator	150,178	stem_loop, feature/stem_loop

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1994017/1

Member of these Collections

• //function/crispr/cas9
• iGEM Parts Registry