pLambda

BBa_K200012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K200012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kun Xue

Date created: 2009-08-30 11:00:00

Date modified: 2015-05-08 01:11:21

lambda promoter (cIts responsive)

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K200012_sequence (Version 1)

Description

This common lambda promoter is able to be repressed by temperature sensitive cI protein.


This has all 3 operator regions of the lambda promoter, as opposed to only operator region 1 and 2 for the registry promote (BBa_R0051)


This promoter is chosen as it has been shown in bacterial lysis experiments lp1 to be stringently repressed by the temperature sensitive cI protein(BBa_K200011) at temperatures up to 30??C.


At temperatures higher than 30??C, the cI857 repressor is denatured and unable to bind to the promoter. As a result, promoter activity is induced.


The cI protein-lambda promoter pair provide an effective system for temperature sensitive regulation of gene expression.

Notes

This was pieced together from 2 oligonucleotides.

Source

Source organism is bacteriophage lambda. Sequence taken from lysis plasmid pAW12

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
OR3	1,17	feature/operator, operator
OR2	24,40	operator, feature/operator
OR1	48,64	feature/operator, operator

Other Properties

• igem#experience: None
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K200012/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //promoter
• iGEM Parts Registry