Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: William Cho
Date created: 2016-06-20 11:00:00
Date modified: 2016-10-08 01:16:39
sfGFP + PETase (PET-degrading enzyme, origin I. sakaiensis)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2010004_sequence (Version 1) |
Description
This His-tag is a sequence that allows a conjunct protein to be purified. The tag is composed of repetitions of histidines.Notes
The DNA sequence has been optimized for E. coli K12 and modified to facilitate gBlock synthesis, by avoiding repeated sequences.Source
This sequence is publicly known and was privately modified.| Sequence Annotation | Location | Component / Role(s) |
| T7 Promoter RBS 34 sfGFP Linker PETase GGS Linker His-Tag Start Codon Stop Codon | 1,46 47,58 65,778 779,793 794,1663 1664,1699 1700,1717 65,67 1718,1723 | feature/promoter promoter ribosome_entry_site feature/rbs feature/protein CDS feature/misc sequence_feature feature/protein CDS feature/misc sequence_feature tag feature/tag feature/start start_codon stop_codon feature/stop |