| Types | DnaRegion
|
| Roles | promoter
Regulatory
|
| Sequences | BBa_K202001_sequence (Version 1)
|
Description
was designed according to the Lutz R and Bujard H.(Nucleic Acids Res. 1997 Mar 15;25(6):1203-10). The arrangement of the operator sites are the same as in BBa_K202000 except that tetO2 and LacO1 opeartor sequences are replaced by two nonbinding sequences (obtained from noncoding sequences of Fungus). This promoter has tetO2 sites which are having transverse mutation at position 3. The hybrid promoter is cloned upstream to the GFP (part Bba_E0240) in plasmid pSBA1. The construct is ready for promoter assay.
Notes
The regulatory architecture is designed such that each operator's position efficiently interferes with RNA polymerase (RNAp) promoter binding without inhibiting promoter function.
Source
Part was designed from tetO2, lambda phage and intervening sequences of the fungus.
