Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
Date created: 2016-10-06 11:00:00
Date modified: 2016-10-19 05:31:27
pTAC cI-GFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K2066031_sequence (Version 1) |
Description
This part was designed to be assembled via Gibson Assembly from WM16_015 (using primers WM16_P014 and WM16_P009) and the same WM16_015 again (using primers WM16_P019 and WM16_P011).This part follows Lou et al. 2012 (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???). This part will be used in concert with WM16_026 (pTAC Ribozyme Characterization Part ???GFP Only; no RiboJ) to determine the influence of the RiboJ insulator sequence on gene expression.
Source
The sequence of this part is modified from Lou et al. (2012) and was synthesized using parts from the iGEM registry as well as UNS spacer sequences from Torella et. al (2013).| Access | Instance | Definition |
| public public public public public public public | BBa_K2066018 BBa_K864400 BBa_K2066528 BBa_K2066515 BBa_K2066511 BBa_B0015 BBa_K2066019 | BBa_K2066018 BBa_K864400 BBa_K2066528 BBa_K2066515 BBa_K2066511 BBa_B0015 BBa_K2066019 |